Isolation of Gram -ve Bacteria from the Sewage Water and Urine Samples, Synthesis & Characterization of Sodium Nitrate Nano Particles using UV- Visible Spectroscopy and Determination of UV Decimal Death Time of Gram -ve Bacteria P. vulgaris, P.mirabilis a

Abstract

We have isolated gram -ve bacteria Enterobacter aerogenes and Pseudomonas aeruginosa successfully from sewage and urine samples and the bacteria shown to possess swarming motility. Both gram negative bacteria Enterobacter aerogenes and Pseudomonas aeruginosa has shown negative tests for Indole test and methyl red test and positive for citrate utilisation test. Studies on antimicrobial activity using Enterobacter aerogenes proved to possess antifungal properties and inhibit the growth of fungus strain C.tropicalis isolated from mouth washings. Klebsiella pneumoniae forms combined biofilms with Candida tropicalis and responsible for fungal abundance appearance on surface of agar plate. C.tropicalis can be able to grow on chromogenic substrates like PNPP and bile pigments due to presence of enzyme alkaline phosphatase and can survive in stools of humans. Sodium nitrate nano particles are synthesized and characterized using U.V visible spectrophotometer through a scan with range of 260nm to 360nm and the decimal death rate of bacteria P. vulgaris, P.mirabilis and K.pneumoniae were calculated accordingly and graphically represented with 7 minutes in case of P. mirabilis and K. pneumoniae and 5 minutes for P.vulgaris