Pharmacological Potential of Gynura Procumbens Root Extract: Role in Oxidative Stress, Inflammation and Nephroprotection

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Priyanka Sukdev Pagare,Chainesh Shah ,Shahzad Ahmed A. R

Abstract

This study assessed the nephroprotective, anti-inflammatory, and antioxidant properties of Gynura procumbens methanol root extract (GPME-R). After the plant material was extracted, it underwent tests to determine its total phenolic content, reducing power, ability to scavenge radicals such as DPPH and superoxide, and ability to chelate metals. It was also determined whether the extract inhibited the enzymes 5-lipoxygenase (LOX), cyclooxygenase-1 (COX-1), and cyclooxygenase-2 (COX-2). Gynura procumbens root extract's nephroprotective potential was also assessed in HEK-293 cells exposed to cisplatin-induced damage. The extract has 0.177 mg/mL of gallic acid equivalents of phenolic compounds, according to the results. The extract showed less action in the reducing power assay at all doses when compared to quercetin and vitamin C. The extract was less effective than BHT for DPPH radical scavenging at higher doses, but more effective at lower concentrations. COX-1, COX-2, and LOX were all shown to be inhibited by the extract in a dose-dependent manner; LOX was most significantly inhibited (IC50 of 101.88 μg/mL). There was also notable metal chelating activity in the extract. Additionally, the extract showed notable dose-dependent nephroprotective efficacy in cisplatin-induced nephrotoxicity model in HEK-293 cells. According to these results, GPME-R has strong nephroprotective, anti-inflammatory, and antioxidant characteristics.

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