Exploring the Indigenous Microbes from Deliming Effluent to reduce heavy metals for greener approach

Abstract

Bioremediation is an affordable, green technique that makes dealing with heavy metal toxicity simple. From the deliming effluent, five isolates were found. With respect to the heavy metal levels up to 5000 ppm, the MICs for each isolate varied. Bacillus cereus B66 OR740559, Bacillus amyloliquefaciens BAB-807 OR740568 and Bacillus rugosus SPB7 OR740574 were the high resistance isolates that 16S rRNA was able to identify. Using Atomic Absorption Spectroscopy (AAS), the reduction capacity was evaluated. Maximum reduction of Bacillus cereus B66 showed maximum reduction at 37℃ pH 9 Pb 92%, Cu 92%. Bacillus amyloliquefaciens BAB-807 was seen at 37°C and pH 9 Pb 90%, Cu 90%; at 45°C and pH 9 Pb, Cu, 92%, 93% during 48 hours. However, in 48 hours, 93% of the Pb and Cu were reduced at 55°C in pH 9. The three most important variables were temperature, pH, and incubation time (p < 0.0001). Among top three isolates, finding indicates that, for the longest possible exposure duration, Bacillus cereus B66 & Bacillus amyloliquefaciens BAB-807 reduction is most effective at temperatures of 37°C, 45°C,55°C with an increase in pH. These findings could serve as a foundation for the development of bioremediation techniques for the treatment of tannery effluent.