“Anti-Inflammatory Effects and EDX Spectra Analysis of Zinc Oxide Nanoparticles Synthesized Using Phoenix dactylifera Extracts: An In Vitro Study ”

Abstract

Aim: The goal of this project was to synthesize and characterize zinc oxide (ZnO) nanoparticles using an aqueous extract of Phoenix dactylifera and to evaluate their anti-inflammatory properties. Methods:Using an aqueous extract of Phoenix dactylifera as the reducing agent, ZnO nanoparticles were produced. Utilizing Energy-Dispersive X-ray (EDX) spectroscopy, characterization was carried out. The anti-inflammatory properties were evaluated through the albumin denaturation assay, where varying concentrations (10µL, 20µL, 30µL, 40µL, and 50µL) of the nanoparticles were tested. Results: EDX analysis confirmed the presence of ZnO nanoparticles, showing 8.5% zinc and 35.2% oxygen by weight. Additional signals for silver, sulfur, and carbon were attributed to biomolecules from the extract. The albumin denaturation assay revealed that the anti-inflammatory activity of the ZnO nanoparticles increased with concentration, achieving maximum effectiveness at 50µL. Discussion: EDX analysis verified that ZnO nanoparticles had formed successfully. The nanoparticles synthesized using Phoenix dactylifera extract demonstrated significant anti-inflammatory properties, consistent with previous findings on ZnO nanoparticles' ability to inhibit mast cell proliferation and block pro-inflammatory cytokines. The peak effectiveness at 50µL suggests a concentration-dependent response. Conclusion: ZnO nanoparticles synthesized using Phoenix dactylifera exhibit notable anti-inflammatory properties, making them promising candidates for therapeutic applications with minimal side effects. Further in vivo studies and comprehensive nanoparticle characterization are recommended to explore their full potential in biomedicine.