Assessment of Production of Biofilm in Urinary Tract Infections in Catheterized Tropical Patients: A Comparative Analysis of Detection Techniques

Abstract

Urinary tract infections, which can be acute or chronic, are quite common in the Indian population. But untreated UTIs can result in a persistent illness that requires hospitalisation. The current study aims to compare three distinct methods of detecting uropathogen biofilm generation in tropical catheterized patients. Data from 500 catheterized tropical patients admitted to the Civil Hospital of Mazagaon, were collected, and screened. According to a predetermined process, all patients were questioned, and all relevant health data was logged in a preset way for easy extraction and interpretation. Samples were taken for testing to determine the isolates' microbial arrangement and the location of Biofilm generation. The identification of microorganisms and biofilms was done using conventional methods. Three methods viz, the tube adhesion method, Congo-red agar method modified, and the tissue culture plate method - were each used to identify the formation of a biofilm. E. coli is the highest prevalent urinary pathogen in the catheterized patients in the current study population of patients, according to the findings, which accounts for urinary tract infections. The most abundant and effective biofilm producers among these clinical isolates were demonstrated to be the clinical isolates containing resistant strains of  E. coli. The most reliable techniques for spotting the emergence of biofilms in this patient population were found to be the Tube Adherence Method, Tissue Culture Plate Method, and Modified Congo Red Agar Method, particularly when Resistant E. coli was the etiological agent.