Microalgae Chlorella Vulgarisisolation and GCMS Analysis of Phytocomponent Methanolic Extracts

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Ritabrata Koley, Belarani Mahato,Kuntal Hazra, Afsona Khatun, Rajshekhar Ghorai, Anupam Adhikari, Biplab Debnath Shaileyee Das

Abstract

This study focuses on the isolation of Chlorella vulgaris, a unicellular microalga with therapeutic and nutritional properties, from a freshwater pond in West Bengal, India. The methanolic extract of C. vulgaris was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) to identify its phytochemical components. Eight fatty acid methyl esters were identified, with compounds 4, 5, 6, and 7 being the major constituents. Compound 4 exhibited properties such as preventing hair loss and acting as an antioxidant, while compound 7 showed hepatoprotective, anti-inflammatory, and anti-cancer properties. Compound 5 displayed antifungal and antioxidant activities, while compound 6 showed anti-inflammatory and cholesterol-lowering effects. Compound 2 was also identified, known for its presence in various oils. This study sheds light on the diverse bioactive compounds presents in C. vulgaris, highlighting its potential applications in various fields including medicine and nutrition.

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