Analyzing Phenols and Triterpenoids in Dechaschistia Crotonifolia Leaves Using HPTLC Fingerprinting

Abstract

Utilizing HPTLC fingerprint analysis, it is simple to analyze and understand an extract made up of various phytoconstituents from a qualitative perspective. Dechaschistia crotonifolia (Burn. f.) has been employed in traditional medicine systems for a very long time. Given that HPTLC is a very reliable method of analysis, it was chosen to interpret the phytoconstituents, namely phenols and triterpenoids, in Dechaschistia crotonifolia (Burn. f.), in accordance with Current Good Manufacturing Practices, which emphasize the importance of quality in regard to phytoconstituents. This study uses the CAMAG HPTLC System connected to the Linomat 5 Applicator, TLC Visualizer, and Scanner to create an HPTLC finger print profile for the secondary metabolites, specifically phenols and triterpenoids, in the leaf methanolic extract of Dechaschistia crotonifolia (Burn. f.). Numerous peaks were visible in the chromatogram when phytoconstituents were evaluated using HPTLC densitometric analysis at wavelengths of 254 nm and 366 nm.  The phytochemicals are evaluated by interpreting the peak areas, peak heights, and Rf values that were reported in the appropriate tables. Triterpenoids, which are phytoconstituents, were found in the study. When the Rf values of these compounds are compared with standards as a reference, this information is very helpful in exploring chemical profiling and identifying bioactive ingredients.