In-Vitro and In-Vivo Antibacterial and Antifungal Activity of Juglans-Regia Mediated Silver Nanoparticles against Aspergillus- Ochraceus Induced Toxicity

Abstract

Green Juglans regia (J. regia) leaf extract, It has dual stabilizing and reductant properties, is used in the room-temperature biosynthesis of Ag-NPs. The UV–visible spectrum, zeta potential and microscope imaging using transmission electron were utilized in order to characterize the J. regia-mediatedAgNPs. The formation of J. regia-mediatedAgNPs was analyzed usingUV–vis spectroscopy, which showed that the maximum surface plasmon absorption occurred between 400 and 460 nm. According to the results of the zeta potential study, the extract of J. regia green leaves had a negative value and a rising concentration of J. regia-mediatedAgNPs. Based on TEM image analysis, we know that the average particle size is 31.37 nm, with a standard variation of 7.1 nm. According to the results of the XRD research, the Ag-NPs are crystalline in structure.  Gram-positive and Gram-negative bacteria were tested for the antibacterial activity of J. regia-mediated Ag-NPs using the disc diffusion method. The results showed that the J. regia-mediatedAgNPs had a strong antibacterial activity.  According to the findings presented here, There is great promise regarding Ag-NP usage in a wide range of biomedical and biological research.