Fe3O4 Nanoparticles as Nanozyme for the Quantification of Hydrogen Peroxide using 3-Methyl-2-Benzothiazolinonehydrazone Hydrochloride with N-(1-Naphthyl) Ethylenediamine Dihydrochloride as Chromogenic Probe.

Abstract

A easy and sensitive spectrophotometric method is described for determining the 3-methyl-2-benzothiazolinonehydrazone hydrochloride as the chromogenic co-substrate using a spectrophotometer, the suggested nanozyme approach for detecting hydrogen peroxide in different environment samples, the tolerance limit was determined as the quantity that resulted in a 3% absorbance error in the determination of 2.199 µM of H₂O₂. Less interference was observed for various metal ions. However, it was discovered that several potent oxidizing or reducing species interfered with the suggested technique. the Results revealed that most of the common ions of water constituents have lesser effect on the determination of H2O2 in Water samples, after a day of precision study it is demonstrated that the relative standard deviation values within a day ranged from 2.09 to 7.04%. According to the average of five measurements made over five days, the relative standard deviation of the different H₂O₂ between-day values ranged from 1.56 to 5.9%.