Effect of Cassia obtusifolia L. seeds extracts formulated in ajwa date seeds oil on Imiquimod (IMQ)-induced psoriasis

Abstract

The plant Cassia obtusifolia L. is traditionally demanded to be advantageous in the action towards many dermatological ailments and date seed oil has skin protective effects. The current study aimed to evaluate the anti-psoriatic activity of a newly formulated ointment of ethanol extract of C. obtusifolia seeds in date seeds oil by using Imiquimod-induced Psoriasis in the thin skin of adult albino rats. In the psoriasis model, 0.1% Imiquimod (IMQ) was topically applied on the earlobe and shaved back skin of adult albino rats which tempted swollen scaly skin abrasions similar to plaque-like psoriasis. The 0.2%w/v ethanol seed extract of C. obtusifolia (CODOB) was used to prepare ointment with a combination of 5% v/v date seed oil and tested for pH, viscosity, spreadability, consistency, stability, sensitivity, irritation, and anti-psoriasis effects. Topical use of formulated ointment with and without date seed oil and plain date seed oil significantly (p < 0.01) subdued the symptoms of psoriasis after IMQ application. From the literature and chemical tests, phenolic compounds, unsaturated fatty acids, and phytosterols have been identified as the major components of C. obtusifolia seeds whereas steroids, fat-soluble vitamins, fatty acids, arachnoids, and different types of fatty esters are identified in date seed oil by GC-MS analysis. From this study, we determined that the topical use of formulated ointment with date seed oil has potent anti-psoriatic activity, and oil from date seeds can be utilized as appurtenant for the treatment of psoriasis.