DNA Sequencing of Aspergillus nomiae Isolated from Sorghum bicolor: Insights via Polymerase Chain Reaction

Abstract

This paper provides a thorough genetic analysis of Aspergillus nomiae strains isolated from Sorghum bicolor using PCR to study their genetic diversity and pathogenicity. Through a thorough literature review, we established the significance of A. nomiae in agricultural ecosystems and its potential impact on Sorghum bicolor, a vital cereal crop worldwide. The utilization of PCR allowed for targeted amplification of conserved regions within the fungal genome, enabling precise identification and differentiation of A. nomiae strains. Our results revealed a diverse genetic landscape among the isolated A. nomiae strains, indicating the existence of distinct genotypes with potential variations in virulence factors. Comparative analysis with existing genomic data sets highlighted unique genomic signatures, shedding light on potential adaptations and evolutionary pathways within A. nomiae populations. Furthermore, phylogenetic analysis elucidated the evolutionary relationships between the isolated strains and other Aspergillus species, providing valuable insights into the taxonomy and evolutionary history of A. nomiae. This research contributes to a deeper understanding of the genetic diversity within A. nomiae populations and underscores the importance of molecular techniques like PCR in studying fungal pathogens.