Biopotential Extraction of Methanol Extract from the Leaves of Persicaria Hydropiper with an Emphasis on Cytotoxicity and Lipid Peroxide Inhibition

Abstract

This comprehensive study examined the properties of microwave-assisted extract of Persicaria hydropiper leaves (MAEPH), a traditionally used medicinal plant, with a focus on its total flavonoid content, antioxidant activity, lipid peroxide inhibition, and cytotoxic effects on MCF7 cells. The results shed light on the possible medicinal applications and advantages of MAEPH. A reliable regression equation was used to precisely measure the crucial parameter known as total flavonoid content, proving that MAEPH is a rich source of these strong antioxidants. The fact that MAEPH displayed concentration-dependent nitric oxide scavenging action suggests that it has the potential to reduce inflammation and oxidative stress, both of which are related to a number of health issues. Through IC₅₀ values and comparisons with ascorbic acid, its effectiveness was further demonstrated. By significantly inhibiting the production of lipid peroxide in both goat liver and egg yolk models, MAEPH's strong antioxidant activities were validated, showing its ability to protect cellular membranes and lipids from oxidative damage. Notably, the study investigated the cytotoxicity of MAEPH on MCF7 cells, revealing dose-dependent effects with a calculated IC₅₀ value, suggesting its potential to inhibit breast cancer cell proliferation. Although MAEPH's cytotoxicity may not be as high as that of the traditional cancer and cytotoxic drug Cyclophosphamide, its lower toxicity highlights its potential as a more bearable option. In conclusion, this study highlights MAEPH's potential as a natural source of antioxidants and a possible candidate for cancer treatment. These results lay a solid groundwork for future research, including in vivo studies, into the health advantages and possible uses of MAEPH. This study offers fresh opportunities for the creation of medicines and substances that promote health, making contributions to the fields of natural medicine and pharmaceutical sciences.